Which is the final step in the described kiwi DNA isolation protocol?

Which is the final step in the described kiwi DNA isolation protocol?

• A. Chop Kiwi
• B. Mix washing up liquid and salt in water
• C. Add kiwi pieces to mixture
• D. Blend mixture for 3 seconds

Answer: (D)

The essential idea is to release DNA from the cells by both chemical and mechanical disruption. Chopping the kiwi increases surface area so the detergent solution can act on more cells. The mixture of washing-up liquid and salt in water provides the chemical environment: the detergent dissolves the cell membranes and nuclear envelopes, while the salt helps to stabilize and loosen DNA so it can be released into solution. Adding kiwi pieces to this mixture starts the breakdown, but a brief blend is the final step that provides the needed mechanical force to break open the remaining cells and tissue quickly, releasing most of the DNA into the liquid. Doing this for only a short time helps release DNA efficiently without cutting it into very small fragments, which could happen with longer blending.

The essential idea is to release DNA from the cells by both chemical and mechanical disruption. Chopping the kiwi increases surface area so the detergent solution can act on more cells. The mixture of washing-up liquid and salt in water provides the chemical environment: the detergent dissolves the cell membranes and nuclear envelopes, while the salt helps to stabilize and loosen DNA so it can be released into solution. Adding kiwi pieces to this mixture starts the breakdown, but a brief blend is the final step that provides the needed mechanical force to break open the remaining cells and tissue quickly, releasing most of the DNA into the liquid. Doing this for only a short time helps release DNA efficiently without cutting it into very small fragments, which could happen with longer blending.