How many steps are listed in the genetic engineering process described, including isolation and expression?

How many steps are listed in the genetic engineering process described, including isolation and expression?

• A. 4
• B. 5
• C. 6
• D. 3

Answer: (B)

The question is testing how to count the major stages in a genetic engineering workflow. When isolation of the gene and its expression are both included, the described process typically breaks into five distinct stages: isolating the gene of interest, inserting it into a vector, introducing the recombinant vector into host cells, screening or selecting cells that carry the construct, and expressing the gene to produce the desired product. Sub-steps like cutting and ligating DNA are usually considered part of the insertion step, not separate steps in the count. So five is the best answer because it reflects five clear stages in the described sequence. A smaller number would merge two stages, while a larger number would imply extra steps not listed as separate stages in the description.

The question is testing how to count the major stages in a genetic engineering workflow. When isolation of the gene and its expression are both included, the described process typically breaks into five distinct stages: isolating the gene of interest, inserting it into a vector, introducing the recombinant vector into host cells, screening or selecting cells that carry the construct, and expressing the gene to produce the desired product. Sub-steps like cutting and ligating DNA are usually considered part of the insertion step, not separate steps in the count. So five is the best answer because it reflects five clear stages in the described sequence. A smaller number would merge two stages, while a larger number would imply extra steps not listed as separate stages in the description.